常见限制性酶切位点 最低0.27元开通文库会员，查看完整内容>；原发布者：sxwfj常见限制性内切酶2113识别序列(酶切位点5261)（BamHI、EcoRI、HindIII、NdeI、XhoI等）分4102子生物学实验室技术2009-11-1717：46：32阅读2235评论2字号：大中1653小订阅.在分子克隆实验中，限制性内切酶是必不可少的工具酶。无论是构建克隆载体还是表达载体，要根据载体选择合适的内切酶（当然，使用T载就不必考虑了）。先将引物设计好，然后添加酶切识别序列到引物5’端（英语怎么说？常用的内切酶比如BamHI、EcoRI、HindIII、NdeI、XhoI等可能你都已经记住了它们的识别序列，不过为了保险起见，还是得查证一下。下面，我就总结了一些常用的内切酶的识别序列，仅供各位参考。下面这些内切酶都属于II型内切酶。先介绍一下什么是II型内切酶吧。TheTypeIIrestrictionsystemstypicallycontainindividualrestrictionenzymesandmodificationenzymesencodedbyseparategenes.TheTypeIIrestrictionenzymestypicallyrecognizespecificDNAsequencesandcleaveatconstantpositionsatorclosetothatsequencetoproduce5-phosphatesand3-hydroxyls.UsuallytheyrequireMg2+ionsasacofactor，althoughsomehavemoreexoticrequirements。.
限制性内切酶保护碱基表 最低0.27元开通文库会员，查看完整内容>；原发布者：傀儡加冕者PCR设计引物时酶切位点2113的保护碱基表5261ApaI（类型：TypeIIrestrictionenzyme）识别4102序1653列：5'GGGCC^C3'BamHI（类型：TypeIIrestrictionenzyme）识别序列：5'G^GATCC3'BglII（类型：TypeIIrestrictionenzyme）识别序列：5'A^GATCT3'EcoRI（类型：TypeIIrestrictionenzyme）识别序列：5'G^AATTC3'HindIII（类型：TypeIIrestrictionenzyme）识别序列：5'A^AGCTT3'KpnI（类型：TypeIIrestrictionenzyme）识别序列：5'GGTAC^C3'NcoI（类型：TypeIIrestrictionenzyme）识别序列：5'C^CATGG3'NdeI（类型：TypeIIrestrictionenzyme）识别序列：5'CA^TATG3'NheI（类型：TypeIIrestrictionenzyme）识别序列：5'G^CTAGC3'NotI（类型：TypeIIrestrictionenzyme）识别序列：5'GC^GGCCGC3'SacI（类型：TypeIIrestrictionenzyme）识别序列：5'GAGCT^C3'SalI（类型：TypeIIrestrictionenzyme）识别序列：5'G^TCGAC3' SphI（类型：TypeIIrestrictionenzyme）识别序列：5'GCATG^C3'XbaI（类型：TypeIIrestrictionenzyme）识别序列：5'T^CTAGA3'XhoI（类型：TypeIIrestrictionenzyme）识别序列：5'C^TCGAG3'
常见限制性内切酶识别序列(酶切位点) 最低0.27元开通文库会员，查看完整内容>；原发布者：稼腾Q7759TheTypeIIrestrictionsystemstypicallycontainindividualrestrictionenzymesandmodificationenzymesencodedbyseparategenes.TheTypeIIrestrictionenzymestypicallyrecognizespecificDNAsequencesandcleaveatconstantpositionsatorclosetothatsequencetoproduce5-phosphatesand3-hydroxyls.UsuallytheyrequireMg2+ionsasacofactor，althoughsomehavemoreexoticrequirements.Themethyltransferasesusuallyrecognizethesamesequencealthoughsomearemorepromiscuous.ThreetypesofDNAmethyltransferaseshavebeenfoundaspartofTypeIIR-MsystemsformingeitherC5-methylcytosine，N4-methylcytosineorN6-methyladenine.ApaI（类型e79fa5e98193e4b893e5b19e31333433623830：TypeIIrestrictionenzyme）识别序列：5'GGGCC^C3'BamHI（类型：TypeIIrestrictionenzyme）识别序列：5'G^GATCC3'BglII（类型：TypeIIrestrictionenzyme）识别序列：5'A^GATCT3'EcoRI（类型：TypeIIrestrictionenzyme）识别序列：5'G^AATTC3'HindIII（类型：TypeIIrestrictionenzyme）识别序列：5'A^AGCTT3'KpnI（类型：TypeIIrestrictionenzyme）识别序列。
